.Silver Nanoparticles Attenuate Inflammation aggravation in Hepatocellular Carcinoma in Rats.

Document Type : Original Article

Authors

1 Radiation Biology Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt

2 Biochemistry department, faculty of science, Ain Shams University, Cairo, Egypt

3 Chemistry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority (AEAE), P.O. Box 29, Nasr City 11731, Cairo, Egypt

4 Department of Radiation Biology, National Center of Radiation Research and Technology, Atomic Energy Authority, Egypt

Abstract

Using silver nanoparticles is a promising tactic in healthcare and cancer therapy, either alone or as an adjuvant in traditional chemotherapy. Also, using gamma radiation in nanoparticle preparation is simple, powerful, and environmentally friendly. This study aimed to assess the impact of silver nanoparticles (AgNPs) prepared by gamma radiation (γ-radiation) on hepatocellular carcinoma (HCC) induced by diethyl-nitrosamine (DEN). MTT Cytotoxicity assay of AgNPs has been detected in vitro on Wi38 normal fibroblast and HepG2 hepatocellular carcinoma cell lines. Twenty-eight adult male albino rats were divided into four groups. After HCC development, rats received (10µg/g b. wt.) of AgNPs, three times a week for four weeks liver enzymes were investigated. TNF-α, TGF-β, and IL-1β inflammatory markers were detected. Also, the transcriptional factor (NF-κB) was calculated. The obtained results confirmed the selective influence of AgNPs on the HCC cell line in a time and dose-dependent manner. Moreover, the role of AgNPs is documented in diminishing the inflammation accompanied by HCC development through decreased inflammatory markers and increased apoptosis.

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