Nano-Selenium Effect on Sexual Hormones and Enzymatic Activity in Relation to Sexual Puberty in NZW Rabbits

Document Type : Original Article

Authors

1 Biological Applications Departement, Nuclear Research Center, Cairo, Egypt

2 Biological Applications Department, Radioisotopes Applications Division, Nuclear Research Centre, Egyptian Atomic Energy Authority, Inshas13759, Cairo, Egypt.

3 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, China.

4 Biological Applications Department, Radioisotopes Applications Division, Nuclear Research Center, Atomic Energy Authority, Inshas13759, Cairo, Egypt.

5 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, Huazhong Agricultural University, Wuhan 430070, China

Abstract

This study aimed to investigate the impact of selenium nano form (Nano-Se) and sodium selenite (SSe) on sexual hormones and enzymatic activity of New Zealand White (NZW) rabbits at puberty. Thirty-six male rabbits and the same number of females (6-weeks old) were distributed into three groups of 24 animals (12 females and 12 males); the groups injected intramuscularly (i.m.) once a week with distilled-water (4ml) as control, 4 ml of a solution containing 90 µg/kg live body weight of Nano-Se or SSe, respectively, for eight weeks. Serum, testicles, and ovaries were obtained at the end of the experiment. Nano-Se increased (P<0.01) ovarian and serum activities of lipase, amylase, and albumin (alb) concentration, with a decrease (P<0.01) in alkaline phosphatase (ALP) activity. In contrast, it decreased ovarian tissue activity of creatine phosphokinase (CPK) and serum alpha-fetoprotein (AFP) and increased (P<0.01) serum ALP in comparison with control. Nano-Se increased (P<0.01) testicular amylase, lipase, AFP, serum CPK, and alb (an opposite trend observed in SSe treatment), with a decrease (P<0.01) of serum ALP and alb. SSe increased (P<0.01) ovarian amylase, CPK, ALP activities, alb, and serum ALP. However, SSe decreased (P<0.01) blood amylase, lipase (also in ovarian tissue), CPK, and AFP weighed to control. Nano-Se and SSe raised Se concentrations in ovarian and testicular extract compared to control. Estradiol-17β, progesterone, and testosterone increased (P<0.01) due to Nano-Se taking

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