Protective Effect of Emblica officinalis on Cardiac Dysfunction in Gamma-Irradiated Male Albino Rats

Document Type : Original Article

Authors

1 zoology departement, faculty of science, Ain shams university

2 radiation biology, national center for radiation research and technology

3 radiation biology, national center for radiation research and technology, atomic energy authority

Abstract

Abstract: Emblica officinalis (EO) is a plant with diverse ethnical medicinal uses. The plant has been explored for diverse pharmacological actions, here it was planned to screen the radio-protective activities of its fruit powder. Experimental Animals and Design: Four groups of male albino rats were used: 1- Control: untreated group. 2- Irradiated: animals exposed to a single dose of whole-body gamma-irradiation (6Gy). 3- Eo group: given daily EO fruit powder dissolved in distalled water at a dose of 750mg/kg b.wt., for 30 days, intragastrically. 4- EO+ Irrad. group: given EO (as group 3), the last dose being 2 hours before irradiation. Blood and heart tissue samples were collected after 2hrs and 2 weeks post irradiation. Parameters Measured: Reduced glutathione (GSH) content and malondialdehyde (MDA) levels were measured in cardiac mitochondrial fraction. Xanthine oxidase (XO) and xanthine dehydrogenase (XDH) activities and the level of advanced oxidation protein products (AOPP) were measured in the cytosolic fraction of heart tissue. Atrial natriuretic peptide (ANP) and creatine phosphokinase (CPK) activity were measured in plasma. Cardiac cytosolic glucose-6-phosphate dehydrogenase (G6PDH) and Lactate dehydrogenase (LDH) activities were determined. Results: EO administration pre-irradiation, improved the disturbances induced in the heart by irradiation.

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