Green synthesis of Spherical Gold Nanoparticles by Chitosan for 6 Mercaptopurine Delivery

Document Type : Original Article

Authors

1 lecturer assistant at NILES,Cairo university

2 professor in laser science and interaction at NILES,cairo university

3 Cancer Biology Department, National Cancer Institute, Cairo University

4 Laser science and interaction, National Institute of Laser Enhanced Sciences, Cairo University

Abstract

In our present work gold nanoparticles were successfully prepared using chitosan as reducing and capping agent and subsequently used as carriers for hydrophobic anticancer 6 mercaptopurine (6MP). The use of this drug is limited due to its poor bioavailability. The purpose of the present investigation was to evaluate a method for loading hydrophobic drug 6MP on chitosan reduced gold nanoparticles (AuNPs).The resulting AuNPs and 6MP loaded AuNPs were characterized by ultraviolet visible spectrophotometry, transmission electron microscopy. To estimate the improvement of the drug delivery of AuNPs, breast cancer cells MCF7 were treated with 6MP and 6MP loaded AuNPs and cell viability was studied in vitro using Sulphorhodamine-B (SRB) assay. The data showed, both free 6MP and 6MP loaded AuNPs revealed anti-proliferation activity against the cancer cells in dose-dependent manners. Moreover, 6MP loaded AuNPs displays significantly greater anti-proliferation activity than that of free 6MP at all concentrations and IC50 1.93nM. The improvement of anti-proliferation activity of 6MP loaded AuNPs compared to that of 6MP can be attributed to enhanced intracellular uptake of the functionalized GNPs via the mechanism of endocytosis

Keywords

Files

Share

How to cite

Statistics